Leukocyte Diapedesis

Biomedical Process Illustration

Leukocytes are immune cells in the bloodstream that can be recruited to the inflammatory regions where pathogens are. The process that leukocytes cross the epithelial cells of a capillary blood vessel is called leukocyte diapedesis. This is a fundamental concept in immunology and the following illustration present this biomedical process in a textbook didactic style. 

Guo_Mimi_MSC2009_2021_Leukocyte Diapedesis.png

Faculty advisor

Dave Mazierski and Shelley Wall

Medium/Software

Adobe Illustrator

Final presentation format

Textbook illustration

Primary audience

Undergraduate immunology students

Work Process

1. Ideation, Reference, and Rough Drafts

After reading on the topic, a rough layout was drafted with selected information. The main image depicts the steps of leukocyte diapedesis. The side image introduces the molecular receptors involved in the process. 

Leukocyte diapedesis-draft-Mimi-Jan25.png

Reference

  1. Kolaczkowska, E., & Kubes, P. (2013). Neutrophil recruitment and function in health and inflammation. Nature Reviews Immunology, 13(3), 159–175. https://doi.org/10.1038/nri3399

  2. Carman, C. V. (2009). Mechanisms for transcellular diapedesis: probing and pathfinding by `invadosome-like protrusions’. Journal of Cell Science, 122(17), 3025–3035. https://doi.org/10.1242/jcs.047522

  3. Filippi, M.-D. (2016). Mechanism of Diapedesis. Advances in Immunology, 25–53. https://doi.org/10.1016/bs.ai.2015.09.001

  4. Petri, B., & Bixel, M. G. (2006). Molecular events during leukocyte diapedesis. FEBS Journal, 273(19), 4399–4407. https://doi.org/10.1111/j.1742-4658.2006.05439.x

  5. Immunology - Innate Immunity (Diapedesis). (2012, February 15). [Video]. YouTube. https://www.youtube.com/watch?v=jUecrQqcx7k

  6. Lumen Learning. (2012). Leukocytes and Platelets | Anatomy and Physiology. https://courses.lumenlearning.com/nemcc-ap/chapter/leukocytes-and-platelets/

  7. F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s7.ogv. (2016). [SEM image]. https://commons.wikimedia.org/w/index.php?title=File%3AF-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s7.ogv

  8. Acute Inflammation- Educational 3D Animation. (2016, August 23). [Video]. YouTube. https://www.youtube.com/watch?v=1SvEdg94qUA&feature=emb_logo

  9. Neutrophils: Symptoms/Causes For Variation & What You Can Do About It. (2017, January 14). Grow Fit. https://getgrowfit.com/blogs/grow-fit-blog/neutrophils-symptoms-causes-for-variation-what-you-can-do-about-it

  10. Paxton, S. (2003). Blood: The Histology Guide. The Histology Guide. https://www.histology.leeds.ac.uk/blood/blood_wbc.php

  11. PromoCell. (2019, December 16). Endothelial Cells - What You Should Know. https://www.promocell.com/cell-culture-basics/endothelial-cells/

  12. Bershadsky, A. D., & Kozlov, M. M. (2011). Crawling cell locomotion revisited. Proceedings of the National Academy of Sciences, 108(51), 20275–20276. https://doi.org/10.1073/pnas.1116814108

  13. How Cells Crawl. (2013, September 4). NIH Intramural Research Program. https://irp.nih.gov/catalyst/v21i5/how-cells-crawl

  14. Carman, C. V., & Springer, T. A. (2004). A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. Journal of Cell Biology, 167(2), 377–388. https://doi.org/10.1083/jcb.200404129

  15. Petri, B., Kaur, J., Long, E. M., Li, H., Parsons, S. A., Butz, S., Phillipson, M., Vestweber, D., Patel, K. D., Robbins, S. M., & Kubes, P. (2011). Endothelial LSP1 is involved in endothelial dome formation, minimizing vascular permeability changes during neutrophil transmigration in vivo. Blood, 117(3), 942–952. https://doi.org/10.1182/blood-2010-02-270561